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Primary Antibodies
IRF3 Antibody
Background
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-α/β genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.
Additional Names
IRF3
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Description
Left: Western blot analysis of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 μg/ml.
Below: Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 μg/ml.
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Other Product Images
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Source
IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IRF3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IRF3 (Intermediate Domain) Peptide (Cat. No. 3399P)
Application
IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3399P - IRF3 Peptide
Storage
IRF3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1215 - U937 Cell Lysate
- Cat. No. 1225 - Ramos Cell Lysate
Species Reactivity
H
Protein GI Number
4504725
Protein Accession Number
NP_001562
Short Description
interferon regulatory factor 3
This product belongs to the following categories:
Related Products
References- Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54.
- Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
- Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
- Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
Datasheet 08-02W
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