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Recombinant Proteins
IRAK Recombinant Protein
Background
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
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Description
Left: SDS-PAGE analysis of recombinant IRAK fragment on Coomassie Blue-stained 12% SDS-PAGE gel.
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Source
E. coli
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Tested Application
E, WB, MS
Buffer
100mM sodium phosphate, 500mM NaCl, 25 mM imidazole, 10% gycerol
Storage
Store at -70˚C. As with any protein, exposing IRAK recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Protein GI Number
8928535
Protein Accession Number
P51617
Domain Information
aa 618-712
Molecular Weight
15 kDa (Calculated)
Fusion Partner
C-terminal His-tag
Purity
~95%
Short Description
E. coli expressed Human IRAK
This product belongs to the following categories:
Related Products
References- Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
- Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.
- Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.
- Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.
Datasheet 08-01W
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