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Detection Sets
IRAK Detection Set
Background
Members of the IRAK (IL-1R-associated kinase)/Pelle family play a major role in IL-1R/TLR mediated inflammatory responses and in innate immunity. IRAK and IRAK-2 regulate the activity of a signaling cascade that mediates the activation of NF-κB and MAP kinase. IRAK-4 interacts with and phosphorylates IRAK, while IRAK-M is thought to inhibit the recruitment and activation of IRAK-4 and IRAK. The importance of the IRAK family in inflammation and immunity is illustrated by the fact that animals lacking IRAK-4 are impaired in their responses to viral and bacterial challenges and are completely resistant to LPS challenge.
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Description
For images please see PDF data sheet
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Kit Contents
IRAK Antibody, Cat. No. 1007 (50μg)
IRAK-2 (C2) Antibody, Cat. No. 2123 (50μg)
IRAK-M Antibody, Cat. No. 2355 (50μg)
IRAK-4 Antibody, Cat. No. 3125 (50μg)
MyD88 Antibody, Cat. No. 2127 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of various IRAK molecules by Western blot at 2 μg/ml to 1 μg/ml and Immunohistochemistry. Whole cell lysate from HeLa cells can be used as a positive control for each antibody except IRAK-M antibody and MyD88 antibody; murine spleen lysate should be used as a positive control for IRAK-M antibody and Jurkat whole cell lysate should be used for MyD88 antibody. For research use only.
Tested Application
WB, IHC
Blocking Peptide
IRAK Peptide, Cat. No. 1007P
IRAK-2 (C2) Peptide, Cat. No. 2123P
IRAK-M Peptide, Cat. No. 2355P
IRAK-4 Peptide, Cat. No. 3125P
MyD88 Peptide, Cat. No. 2127P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -4˚C.
Positive Control
- HeLa Lysate, Cat. No. 1201
- Murine Spleen Lysate, Cat. No. 1406.
- Jurkat Whole Cell Lysate, Cat. No. 1205.
Species Reactivity
see datasheet
Short Description
IRAK, IRAK-2, IRAK-M, IRAK-4 and MyD88
This product belongs to the following categories:
Related Products
References- Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor.Science 1996; 271:1128-31.
- Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediator of IL-1 signaling. Science 1997; 278:1612-5.
- Li S, Strelow A, Fontana EJ, et al. IRAK-4: a novel member of the IRAK family with the properties of an IRAK kinase. Proc. Natl. Acad. Sci. USA 2002; 99:5567-72.
- Kobayashi K, Hernandez LD, Galan JE, et al. IRAK-M is a negative regulator of Toll-like receptor signaling. Cell 2002; 110:191-202.
Datasheet 0803-W
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