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Primary Antibodies
IRAK Antibody
Background
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
Additional Names
IRAK (CT), IL-1-Receptor Associated Kinase
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Description
Left: Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody at 1:2000 dilution.
Below: Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 μg/ml.
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Other Product Images
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Source
IRAK antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
IRAK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IRAK (C-Terminus) Peptide (Cat. No. 1007P)
Application
IRAK antibody can be used for Western blot at 1:1000 dilution and for immunoprecipitation with 2 to 4 ug per sample. An 80 kDa band should be detected in the lysate from non-activated cells. It has no cross response to IRAK2.
Tested Application
E, WB, ICC, IP
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1007P - IRAK Peptide
Storage
IRAK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1208 - THP-1 Whole Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
8928535
Protein Accession Number
P51617
Short Description
(CT) IL-1R associated kinase
This product belongs to the following categories:
Related Products
References- Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996;271:1128-31.
- Trofimova M; Sprenkle AB; Green M; Sturgill TW; Goebl MG; Harrington MA. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J Bio Chem 1996; 271: 17609-1
- Jianing Huang, Xiong Gao, Shyun Li, and Zhaodan Cao. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc Natl Acad Sci USA 1997;94:12829-12832
- Robinson D, Shibuya K, Mui A, Zonin F, Murphy E, Sana T, Hartley SB, Menon S, Kastelein R, Bazan F, O'Garra A. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-γ production and activates IRAK and NF-κB. Immunity 1997;7:571-581
Datasheet 08-02W
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