|
Primary Antibodies
IRAK-4 Antibody
Background
Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κB and MAP kinase (MAPK) by IL-1R/TLR (1-3). A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4 (4,5). Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges (5,6). Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
Additional Names
IRAK-4
|
Description
Left: Western blot analysis of IRAK-4 in HeLa cell lysate with IRAK-4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) μg/ml, respectively.
Below: Immunocytochemistry of IRAK-4 in K562 cells with IRAK-4 antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
IRAK-4 antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IRAK-4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IRAK4 Peptide (Cat. No. 3125P)
Application
IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 2 μg/ml. A 50 kDa band can be detected. Anti-IRAK-4 has no cross response to other IRAKs.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3125P - IRAK-4 Peptide
Storage
IRAK-4 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
20219010
Protein Accession Number
AAM15772
Short Description
A member in IRAK/Pelle family
This product belongs to the following categories:
Related Products
References- Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996;271:1128-31.
- Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5
- Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J Biol Chem. 1999;274(27):19403-10.
- Li S, Strelow A, Fontana EJ, Wesche H. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc Natl Acad Sci USA . 2002;99(8):5567-72.
Datasheet 08-02W
|
|
