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Primary Antibodies
IRAK-2 Antibody
Background
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 . Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
Additional Names
IRAK-2 (C2), IRAK-2
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Description
Left: Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 μg/ml.
Below: Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 μg/ml.
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Other Product Images
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Source
IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human IRAK2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IRAK-2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IRAK2 (C-Terminus 2) Peptide (Cat. No. 2123P)
Application
IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 1-2 μg/ml. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunohistochemistry at 2 μg/ml. Anti-IRAK2 has no cross response to IRAK.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2123P - IRAK-2 Peptide
Storage
IRAK-2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
2653876
Protein Accession Number
AF026273
Short Description
(C2) IL-1R associated kinase
This product belongs to the following categories:
Related Products
References- Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5.
- Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E, Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial lipopolysaccharide activates nuclear factor-κB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 1999;274:7611-4.
- Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 1999;163:639-43. (RD1299)
Datasheet 08-02W
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