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Primary Antibodies
IL-27 Antibody
Background
Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-γ, and T-bet.
Additional Names
IL-27 (IN), Interleukin-27, IL-27 p28
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Description
Left: Western blot analysis of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 μg/ml.
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Source
IL-27 antibody was raised against a 14 amino acid peptide from near the center of human IL-27.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IL-27 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
IL-27 antibody can be used for the detection of IL-27 by Western blot at 2 - 4 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3799P - IL-27 Peptide
Storage
IL-27 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity
H, M
Protein GI Number
28416913
Protein Accession Number
NP_663634
Short Description
(IN) Interleukin 27
This product belongs to the following categories:
Related Products
References- Pfanz S, Timans JC, Cheung J et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity 2002; 16:779-90.
- Pfanz S, Hibbert L, Mattson J, et al. WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27. J. Immunol. 2004; 172:2225-31.
- Hisada M, Kamiya S, Fujita K, et al. Potent antitumor activity of interleukin-27. Cancer Res. 2004; 64:1152-6
Datasheet 08-02W
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