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Primary Antibodies
IL-23 Antibody
Background
Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-γ, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
Additional Names
IL-23 (CT), Interleukin-27, IL-27 p28
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Description
Left: Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunocytochemistry of IL-23 in Raji cells with IL-23 antibody at 10 μg/ml.
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Other Product Images
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Source
IL-23 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human IL-23.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IL-23 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
IL-23 antibody can be used for the detection of IL-23 by Western blot at 2 - 4 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3793P - IL-23 Peptide
Storage
IL-23 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
Species Reactivity
H, M
Protein GI Number
42542809
Protein Accession Number
AAH66268
Short Description
(CT) Interleukin 23
This product belongs to the following categories:
Related Products
References- Hunter CA. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol. 2005; 5:521-31.
- Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 2000; 13:715-25.
- Sheibanie AF, Tadmori I, Jing H, et al. Prostaglandin ED induces IL-23 production in bone marrow-derived dendritic cells. FASEB J. 2004; 18:1318-20.
- Pirhonen J, Matikainen S, Julkunen I. Regulation of virus-induced IL-12 and IL-23 expression in human macrophages. J. Immunol. 2002; 169:5673-8.
Datasheet 08-02W
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