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Primary Antibodies
IKK gamma Antibody
Background
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IkB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1 (1-5). IKKg interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation (3).
Additional Names
IKK gamma (CT),IKKg / NEMO, NEMO, FIP3
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Description
Left: Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/ml.
Below: Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/ml.
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Other Product Images
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Source
IKK gamma antibody was raised against a peptide corresponding to amino acids 400 to 416 of human IKK gamma, which are identical to those of mouse homologue.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IKK gamma antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IKK gamma Peptide (Cat. No. 2335P)
Application
IKK gamma antibody can be used for detection of IKK gamma by Western blot at 0.5 to 1 μg/ml. A 52 kDa band should be detected. IKK gamma has no cross response to IKK alpha or IKK beta.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2335P - IKK gamma Peptide
Storage
IKK gamma antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
3641279
Protein Accession Number
AF074382
Short Description
(CT) IkB Kinase gamma
This product belongs to the following categories:
Related Products
References- Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-γ is an essential regulatory subunit of the IκB kinase complex. Nature 1998;395(6699):297-300
- Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation. Cell. 1998;93(7):1231-40.
- Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-κB activity and as a target of an adenovirus inhibitor of tumor necrosis factor α-induced apoptosis. Proc Natl Acad Sci U S A 1999;96(3):1042-7
- Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. IκB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19(2):1526-38.
Datasheet 08-02W
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