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Primary Antibodies
IKK epsilon Antibody
Background
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. IkB is phosphorylated by IkB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i (1,2). IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1 (1). IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS (2).
Additional Names
IKK epsilon (CT), IKKi, IKKe
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Description
Left: Western blot analysis of IKK epsilon in Jurkat whole cell lysate with IKK epsilon/IKK-i antibody at 1 μg/ml.
Below: Immunohistochemistry of IKK epsilon in human pancreas tissue with IKK epsilon antibody at 10 μg/ml.
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Other Product Images
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Source
IKK epsilon antibody was raised against a peptide corresponding to amino acids 701 to 716 of human IKK epsilon/IKK-i.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IKK epsilon antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IKK epsilon Peptide (Cat. No. 2329P)
Application
IKK epsilon antibody can be used for detection of IKK epsilon by Western blot at 0.5 to 1 μg/ml. An 80 kDa band should be detected. It has no cross response to IKKa, IKKb, or IKKy.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2329P - IKK epsilon Peptide
Storage
IKK epsilon antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1307 - Human Pancreas Tissue Lysate
Species Reactivity
H
Protein GI Number
7288877
Protein Accession Number
AF241789
Short Description
(CT) IkB Kinase epsilon
This product belongs to the following categories:
Related Products
References- Peters RT, Liao SM, Maniatis T. IKK epsilonpsilon is part of a novel PMA-inducible IκB kinase complex. Mol Cell 2000;5(3):513-22
- Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi Y, Kanamaru A, Akira S. IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IκB kinases. Int Immunol 1999;11(8):1357-62 (WD0101)
Datasheet 08-02W
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