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Primary Antibodies
IKK beta Antibody
Background
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for NF-κB activation. IKKβ phosphorylates both IκB-α and IκB-β. IKKβ is expressed in variety of human tissues.
Additional Names
IKK beta (C3), IKK beta, I-kappa-B-kinase beta, I-kappa-B-kinase 2
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Description
Left: Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
Below: Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 μg/ml.
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Other Product Images
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Source
IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IKK beta antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IKK beta (C-Terminus 3) Peptide (Cat. No. 2121P)
Application
IKK beta antibody can be used for detection of IKK beta by Western blot at 1 – 2 μg/ml. A 87 kDa band should be detected. This polyclonal antibody has no cross response to IKKa or IKKy.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2121P - IKK beta Peptide
Storage
IKK beta antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1201 - HeLa Whole Cell Lysate
Species Reactivity
H
Protein GI Number
14285497
Protein Accession Number
O14920
Short Description
(C3) Iκ B kinase β
This product belongs to the following categories:
Related Products
References- DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 1997;388:548-54
- Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IκB kinase. Cell 1997;90:373-83
- Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ, necessary for IκB phosphorylation and NF-κB activation. Cell 1997;91:243-52
- Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IκB kinase-α: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997;278:866-9
Datasheet 08-02W
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