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Primary Antibodies
IKK alpha Antibody
Background
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IΚB degradation and NF-κB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKβ). IKKa and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-α. IKKα is expressed in variety of human tissues.
Additional Names
IKK alpha (C1), IKKa, IKK-1
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Description
Left: Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.
Below: Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1μg/ml.
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Other Product Images
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Source
IKK alpha antibody was raised against a peptide corresponding to amino acids 716 to 734 of human IKK alpha, which differs from corresponding murine sequence by four amino acids .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IKK alpha antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IKK alpha (C-Terminus 1) Peptide (Cat. No. 2025P)
Application
IKK alpha can be used for detection of IKK alpha by Western blot at 1:1000 to 1:2000 dilution. An 85 kDa band should be detected. antibody has no cross response to IKKb or IKKg.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2025P - IKK alpha Peptide
Storage
IKK alpha antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1205 Jurkat Whole Cell Lysate
Species Reactivity
H
Protein GI Number
2327068
Protein Accession Number
AF009225
Short Description
(C1) Iκ B kinase α
This product belongs to the following categories:
Related Products
References- DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 1997;388:548-54
- Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IκB kinase. Cell 1997;90:373-83
- Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ, necessary for IκB phosphorylation and NF-κB activation. Cell 1997;91:243-52
- Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997;278:866-9
Datasheet 08-02W
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