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Primary Antibodies
IGF1R Antibody
Background
Insulin-like growth factor-1 receptor (IGF-1R, also known as CD221), a member of the tyrosine kinase superfamily, is a broadly expressed transmembrane receptor that plays a key role in supporting cell growth and differentiation, and imparts resistance to apoptosis. IGF-1R is synthesized as a single polypeptide that is glycosylated and proteolytically cleaved to yield a disulfide-linked tetrameric receptor composed of two α-subunits and two β-subunits, arranged in the configuration α-β-β-α. IGF-1R’s α-subunits (135 kDa) mediate ligand binding, and are entirely extracellular. IGF-1R’s β-subunits (90 kDa) each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic portion. Three polypeptide ligands for IGF-1R have been identified: IGF-1, IGF-2, and insulin. IGF-1’s binding to the α-subunits of the receptor induces a conformational change, resulting in the trans-autophosphorylation of three tyrosine residues (1131, 1135, and 1136) and activation. Activated IGF-1R phosphorylates substrate proteins, including Shc and insulin receptor substrates (IRS) 1, 2, 3, and 4, and recruits 14-3-3 proteins. This antibody recognizes the β-subunit of IGF-1R, and does not bind to insulin receptor.
Source
IGF1R antibody was produced against recombinant fragment of the cytoplasmic domain of human IGF-1R β-subunit expressed in E. coli.
Clonality / Clone
This is a monoclonal antibody.
Host
IGF1R antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Specificity
Human, mouse and rat.
Application
IGF1R antibody is suitable for use in Western blotting.
Tested Application
WB
Buffer
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Storage
Store at 2-8˚C. For long term storage, aliquot into small volumes and store at –20˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Positive Control
- Human MCF-7 cells, mouse L929 cells and rat PC12 cells.
Species Reactivity
H, M, R
Formulation
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Short Description
IGF1R
This product belongs to the following categories:
References- Amoui, M., et al. (2001) Differential phosphorylation of IRS-1 by insulin and insulin-like growth factor 1 receptors in Chinese hamster ovary cells. J. Endocrinology 171:153-162.
- Blum, G., et al. (2003) Development of new insulin-like growth factor-1 receptor kinase inhibitors using catechol mimics. J. Biol. Chem. 278:40442-40454.
- Favelyukis, S., et al. (2001) Structure and autoregulation of the insulin-like growth factor 1 receptor kinase. Nat. Struct. Biol. 8:1058-1063.
- Peruzzi, F., et al. (1999) Multiple signaling pathways of the insulin-like growth factor 1 receptor in protection from apoptosis. Mol. Cell. Biol. 19:7203-7215.
Datasheet 0801-W
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