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Primary Antibodies
IGF-I Antibody
Background
IGF-I Antibody
Source
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Purification
Anti-hIGF-1 specific antibody was purified by affinity chromatography employing immobilized hIGF-1 matrix.
Clonality / Clone
This is a polyclonal antibody.
Host
IGF-I antibody was raised in rabbit.
Please use rabbit secondary antibodies.
Preparation
A sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.
Application
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-1 (3.00 ng/ml), a concentration of 0.67 0.1 μg/ml of this antibody is required.
ELISA:
To detect hIGF-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-1.
Western Blot:
To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB, N
Storage
IGF-I lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Species Reactivity
H
Short Description
Anti-Human IGF-1
This product belongs to the following categories:
Related Products
Datasheet 1001-W
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