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Primary Antibodies
IEX-1 Antibody
Background
IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-a. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappaB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.
Additional Names
IEX-1, Immediate early response 3, IER3, DIF2, PRG1, GLY96
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Description
Left: Western blot analysis of IEX1 in human brain tissue lysate with IEX1 antibody at (A) 2 and (B) 4 μg/ml.
Below: Immunohistochemistry of IEX-1 in human liver tissue with IEX-1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
IEX1 antibody was raised against a 24 amino acid peptide near the center of human IEX1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IEX-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
IEX1 antibody can be used for detection of IEX1 by Western blot at 2 – 4 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4247P - IEX-1 Peptide
Storage
IEX-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H, M
Protein GI Number
3123229
Protein Accession Number
P46695
Short Description
a stress inducible gene that is induced by ionizing radiation
This product belongs to the following categories:
Related Products
References- Kondratyev AD, Chung K-N and Jung MO. Identification and characterization of a radiation-inducible glycosylated human early-response gene. Cancer Res. 1996; 56:1498-502.
- Feldmann KA, Piddelkow MR, Roche PC, et al. Expression of an immediate early gene, IEX-1, in human tissues. Histochem. Cell Biol. 2001; 115:489–97.
- Wu MX, Zhaohui A, Prasad KVS, et al. IEX-1L, an apoptosis inhibitor involved in NFkappaB mediated cell survival. Science 1998; 281:998-1001.
- Arlt A, Grobe O, Sieke A, et al. Expression of the NF-kappa B target gene IEX-1 (p22/PRG1) does not prevent cell death but instead triggers apoptosis in Hela cells. Oncogene 2001; 20:69–76.
Datasheet 08-02W
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