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Primary Antibodies
ICSBP/IRF8 Antibody
Background
Interferon regulatory factor 8 (IRF8, interferon consensus sequence-binding protein, ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-α and IFN-β. IRF family proteins also control expression of IFN-? and IFN-β-regulated genes that are induced by viral infection.
Additional Names
H-ICSBP, ICSBP, ICSBP1, IRF-8
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Description
Left: Western blot analysis of IRF8 in HepG2 cell lysates using anti-IRF8.
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Purification
Rabbit IgG is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
ICSBP/IRF8 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
ICSBP/IRF8 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human IRF8.
Application
ICSBP/IRF8 antibody can be used for Western blot at a suggested dilution of 0.5-2.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,004 as secondary antibody. The protein size is 426 amino acids.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
ICSBP/IRF8 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ēC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_002154
Molecular Weight
48kDa
Short Description
Anti-Interferon regulatory factor 8 (IRF8)
This product belongs to the following categories:
Related Products
References- Weisz,A., et al., (1992) J. Biol. Chem. 267 (35), 25589-25596
Datasheet 0802-W
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