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Abbreviations
ArArabidopsis
BBovine
BABaboon
BcBacteria
BlBlocking
BtBiotinylated
CeC. elegans
CChicken
ChmChimpanzee
CPCarp
CTC-Terminus
DDog
DsDrosophila
EELISA
EDExtracellular Domain
ELExtracellular Loop
FFeline
FACSFlow Cytometry
FuFungus
GGuinea Pig
GtGoat
HHuman
HMHamster
HrHorse
IImmunogen
ICCImmunocytochemistry
IDIntracellular Domain
IFImmunofluorescence
IHCImmunohistochemistry
INIntermediate Domain
IPImmunoprecipitation
MMouse
MaMammal
MkMonkey
MSMass Spectrometry
MtM. tuberculosis
NNeutralization
NTN-Terminus
PPorcine
PaParasite
PFPuffer Fish
PlPlant
PzProtozoan
RRat
RbRabbit
SSheep
VVirus
WBWestern Blot
XXenopus
YYeast
ZFZebra Fish


Products

ImmunoBlots

Human ImmunoBlots


Human immunoblots from ProSci are made using human cell lines or human tissues. ProSci human immunoblots are designed for quick and easy protein expression screening of multiple human cell lines or human tissues. Each human immunoblot contains nine lanes, including a pre-stained protein marker and each lane is loaded with 15 μg of total protein. However, our immunoblots are highly customizable. Up to 50 μg of protein can be run on virtually any percentage or gradient percentage gel. Human immunoblot membranes can have more than 9 lanes loaded in any order. Our human immunoblots are usually transferred to nitrocellulose, but PVDF membranes may also be requested.

ProSci human immunoblots can be used with any standard membrane procedure. Our human immunoblots can also be reused after stripping off any assay antibodies from the membrane.

Product Cat. # Reactivity App. Description  
Customized Human Normal Tissue Blot 1541N n/a WB Many human normal tissues are available
Customized Human Tumor Tissue Blot 1541T n/a WB Many human tumor tissues are available
Human Cell Line I Blot 1501 n/a WB MCF HeLa HT-29, 293, Hep G2, A-549, SK-N-SH, PC-3
Human Cell Line II Blot 1502 n/a WB Daudi, Raji, K-562, U-937, THP-1, HL-60, Jurkat, MOLT-4
Human Lung Tumor Blot I 1510 n/a WB small, non-small cell differentiated adenocarcinoma, squamous pleomorphic
Human Lung Tumor Blot II 1511 n/a WB A549, small, non-small, squamous cell carcinoma, matched normal lung tissue
Human Lung Tumor Blot IV 1513 n/a WB A549, small cell, differentianted carcinoma, pleomorphic, matched normal lung tissue
Human Lung Tumor Blot V 1514 n/a WB A549, small cell, differentianted carcinoma, pleomorphic, matched normal lung tissue
Human Lung Tumor Blot VI 1546 n/a WB A549, normal lung tissue, small cell carcinoma, non-small cell carcinoma, differentiated adenocarcinoma, squamous cell carcinoma, pleo-morphic carcinoma, non-diagnosed carcinoma, non-diagnosed carcinoma.
Human Normal Tissue Blot I 1521 n/a WB brain, colon, heart, kidney, liver, lung, pancreas, spleen
Human Normal Tissue Blot II 1522 n/a WB brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, spleen.
Human Normal Tissue Blot III 1523 n/a WB colon, duodenum, esophagus, ileum, small intestine, jejunum, pancreas, rectum, stomach.
Human Normal Tissue Blot IV 1524 n/a WB bladder, breast, cervix, kidney, ovary, placenta, prostate, testis, uterus
Human Normal Tissue Blot V 1525 n/a WB appendix, kidney, liver, lymph, node, peripheral, blood, leukocyte, spleen thymus, tonsil, thyroid
Human Normal Tissue Blot VI 1526 n/a WB left, right cerebellum, frontal, occipital, parietal lobe, whole eye, spinal cord, temporal lobe, thalamus.
Human Tumor Tissue Blot I 1542 n/a WB brain, colon, kidney, liver, lung, pancreas, skin, spleen, stomach
Human Tumor Tissue Blot II 1543 n/a WB colon, duodenum, esophagus, small intestine, liver, pancreas, rectum, stomach.
Human Tumor Tissue Blot III 1544 n/a WB bladder, breast, kidney, ovary, prostate, testis, cervix, uterus.
Human Tumor Tissue Blot IV 1545 n/a WB kidney, liver, lymphoma, Non-Hodgkin’s, spleen, thymoma, thyroid, tonsil.

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