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ImmunoBlots
Human Cell Line II Blot
Background
The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are Daudi (B lymphoblast; Burkkit's lymphoma), Raji (B lymphoblast; Burkkit's lymphoma), K-562 (bone marrow; chronic myelogenous leukmia), U-937 (histiocytic lymphoma), THP-1 (monocyte; acute monocytic leukmia), HL-60 (promyeloblast; acute promyeloblastic leukmia), Jurkat (T lymphocyte; acute T cell leukmia), and MOLT-4 (T lymphoblast; acute lymphoblastic leukmia).
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Description
Coomassie blue staining of human cell lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, Daudi; 3, Raji; 4, K-562; 5, U-937; 6, THP-1; 7, HL-60; 8, Jurkat; and 9, MOLT-4.
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Application
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Tested Application
WB
Storage
Store at 4°C. The blot can be reused after stripping.
Species Reactivity
n/a
Short Description
Daudi, Raji, K-562, U-937, THP-1, HL-60, Jurkat, MOLT-4
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Related Products
Datasheet 06-01W
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