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ImmunoBlots
Human Cell Line I Blot
Background
The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are MCF7 (breast; adenocarcinoma), HeLa (cervix; adenocarcinoma), HT-29 (colon; adenocarcinoma), 293 (kidney; transformed with adenovirus 5 DNA), Hep G2 (liver; hepatocellular carcinoma), A-549 (lung; carcinoma), SK-N-SH (brain; neuroblastoma), and PC-3 (prostate;adenocarcinoma).
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Description
Coomassie blue staining of human cell lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, MCF7; 3, HeLa; 4, HT-29; 5, 293; 6, Hep G2; 7, A-549; 8, SK-N-SH; and 9, PC-3.
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Application
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Tested Application
WB
Storage
Store at 4°C. The blot can be reused after stripping.
Species Reactivity
n/a
Short Description
MCF HeLa HT-29, 293, Hep G2, A-549, SK-N-SH, PC-3
This product belongs to the following categories:
Related Products
Datasheet 06-01W
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