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Lysates
Hippocampus Lysate
Background
Human hippocamps tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The human hippocamps tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the hippocamps tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The hippocamps tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Tissue Type
Brain: Hippocampus
Diagnosis
Normal
Source
Human Hippocampus tissue
Application
Hippocampus lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Tested Application
WB
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Human
Short Description
total protein normal hippocampus - human
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Related Products
Datasheet 0802-W
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