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Lysates
Heart Ventricle (LT) (Arrhythmia, infarct) Lysate
Background
Human heart ventricle, left tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The human heart ventricle, left tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart ventricle, left tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart ventricle, left tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Tissue Type
Heart Ventricle, left
Diagnosis
Arrhythmia, infarct
Source
Human Heart Ventricle, left tissue
Application
Heart Ventricle, left lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Tested Application
WB
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Human
Short Description
total protein heart ventricle (lt) (arrhythmia, infarct) - human
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Related Products
Datasheet 0802-W
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