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Slides
HeLa Cell Slide
Background
HeLa S3 (cervix; adenocarcinoma)
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Description
HeLa Cell Slide
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Source
human cervix adenocarcinoma
Preparation
Human HeLa S3 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The Hela cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Hela cells. A follow-up heat dehydration process was done to ensure the attachment of Helacells and the stability of cellular proteins.
Application
Hela cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.
Tested Application
ICC
Storage
Hela cell slides can be stored for up to 6 months at 4°C under desiccate conditions.
Species Reactivity
n/a
Species
Human
Short Description
Human cervix epithelioid carcinoma
This product belongs to the following categories:
Datasheet 0803-W
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