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Slides
HL-60 Cell Slide
Background
HL-60 (promyeloblast; acute promyelocytic leukemia)
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Description
HL-60 Cell Slide
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Source
Human peripheral blood promyeloblast, acute promyelocytic leukemia
Preparation
Human HL-60 cells were cultured in Iscove's modified Dulbecco's medium with 4 mM L-glutamine and harvested at the log phase of growth. The HL-60 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 HL-60 cells. A follow-up heat dehydration process was done to ensure the attachment of HL-60 cells and the stability of cellular proteins.
Application
HL-60 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
Tested Application
ICC
Storage
HL-60 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.
Species Reactivity
n/a
Species
Human
Short Description
Human peripheral blood promyeloblast, acute promyelocytic leukemia
This product belongs to the following categories:
Datasheet 0803-W
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