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Primary Antibodies
HELB Antibody
Background
The protein encoded by this gene is a DNA helicase. A dominant-negative mutant of this protein blocks chromosomal DNA replication and suggests that its function is required for S phase entry.
Additional Names
HELB
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Description
Left: Western blot analysis of HELB in Jurkat Cell Lysate using HELB antibody at 2.5 μg/ml (8% gel).
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Purification
Antibody is purified by protein A chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
HELB antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HELB.
Application
HELB antibody can be used for detection of HELB by ELISA at 1:62500. HELB antibody can be used for detection of HELB by western blot at 2.5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store HELB antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H
Protein Accession Number
NP_387467
Molecular Weight
120kDa
Short Description
HELB (helicase (DNA) B)
This product belongs to the following categories:
References- Taneja,P., et al., (2002) J. Biol. Chem. 277 (43), 40853-40861.
Datasheet 10-01W
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