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Primary Antibodies
HECTD2 Antibody
Background
HECTD2 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Additional Names
HECTD2, FLJ16050
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Description
Left: Western blot analysis of HECTD2 in fetal muscle lysate using HECTD2 antibody at 1 μg/ml (12% gel).
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Purification
Antibody is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
HECTD2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HECTD2.
Application
HECTD2 antibody can be used for detection of HECTD2 by ELISA at 1:312500. HECTD2 antibody can be used for detection of HECTD2 by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store HECTD2 antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M
Protein Accession Number
NP_877497
Molecular Weight
88kDa
Short Description
HECTD2 (HECT domain containing 2)
This product belongs to the following categories:
References- Deloukas,P., (2004) Nature 429 (6990), 375-381.
Datasheet 10-01W
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