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Primary Antibodies
HDAC2 Antibody
Background
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.
Additional Names
RPD3, YAF1
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Description
Left: Western blot analysis of HDAC2 in Jurkat cell lysate using anti-HDAC2.
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Purification
Rabbit IgG is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
HDAC2 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
HDAC2 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human HDAC2.
Application
HDAC2 antibody can be used for Western blot at a suggested dilution of 0.2-2.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,051 as secondary antibody. The protein size is 488 amino acids.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
HDAC2 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001518
Molecular Weight
55 kDa
Short Description
Anti-HDAC2
This product belongs to the following categories:
Related Products
References- Choi, Y.B., et al., (2004) J.Biol.Chem.279(49),50930-50941
Datasheet 0802-W
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