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Primary Antibodies
HAP1 Antibody
Background
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. HAP1 was initially identified through a two-hybrid library screening; the binding of HAP1 to huntingtin correlated with the expansion of the polyglutamine tract. HAP1 also interacts with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), suggesting that HAP1 may play a role in vesicular trafficking or organelle transport. HAP1 is also involved with the huntingtin-enhanced BDNF transport along the cellular microtubles. Attenuation of this process led to the loss of neurotrophic support and neuronal toxicity, which suggests that loss of this function might contribute to pathogenesis. Several alternatively spliced isoforms have been described for HAP1.
Additional Names
HAP1, Huntingtin-associated protein 1, neuroan 1, HLP, HIP5
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Description
Left: Western blot analysis of HAP1 in mouse brain tissue lysate with HAP1 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of HAP1 in human brain tissue with HAP1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
HAP1 antibody was raised against a 19 amino acid peptide from near the center of human HAP1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
HAP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
HAP1 antibody can be used for detection of HAP1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4205P - HAP1 Peptide
Storage
HAP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1403 - Mouse Brain Tissue Lysate
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
10241694
Protein Accession Number
CAC09418
Short Description
Huntingtin associated protein 1
This product belongs to the following categories:
Related Products
References- Borrell-Pagès M, Zala D, Humbert S, et al. Huntington's disease: from huntingtin function and dysfunction to therapeutic strategies. Cell Mol. Life Sci. 2006; 63:2642-60.
- Li X-J, L S-H, Sharp AH, et al. A huntingtin-associated protein enriched in brain with implications for pathology. Nature 1995; 378:398-402.
- Engelender S, Sharp AH, Colomer V, et al. Huntingtin-associated protein 1 (HAP1) interacts with the p150(Glued) subunit of dynactin. Hum. Molec. Genet. 1997; 6:2205-12.
- Gauthier LR, Charrin BC, Borrell-Pages M, et al. Huntingtin controls neurotrophic support and survival of neurons by enhancing BDNF vesicular transport along microtubules. Cell 2004; 118:127-38.
Datasheet 08-02W
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