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Primary Antibodies
HAP1 Antibody
Background
HAP1 Antibody.
Additional Names
Hap1, huntingtin-associated protein 1, RP23-392I3.11, HAP-1, MGC31449
Source
HAP1 antibody was raised against a synthetic peptide near the C-terminus of HAP1.
Purification
HAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
HAP1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 110kDa band in mouse and rat brain lysates after 0.5μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.8kDa according to NP_034534.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E
Buffer
HAP1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-722P - HAP1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
M, R
Protein Accession Number
NP_034534.1
Short Description
(CT) HAP1 (mouse)
This product belongs to the following categories:
Related Products
References- Rong J, McGuire JR, Fang ZH, Sheng G, Shin JY, Li SH, Li XJ. Regulation of intracellular trafficking of huntingtin-associated protein-1 is critical for TrkA protein levels and neurite outgrowth. J Neurosci. 2006 May 31;26(22):6019-30.
Datasheet 08-01W
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