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Primary Antibodies
HAP1 Antibody
Background
HAP1 Antibody. This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
Additional Names
HAP1, huntingtin-associated protein 1 (neuroan 1), HAP2, HIP5, HLP, hHLP1, huntingtin-associated protein 1, huntingtin-associated protein 2, neuroan 1
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Description
Left: Western blot analysis of HAP1 in human hippocampus lysate (35μg protein in RIPA buffer) using HAP1 antibody (0.1μg/mL).
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Source
HAP1 antibody was raised against a synthetic peptide near an internal region of HAP1.
Purification
HAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
HAP1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Approx 70kDa band observed in human brain (frontal cortex and hippocampus) lysates (calculated MW of 69.6kDa according to NP_817084.1). Recommended concentration: 0.1-0.3μg/ml. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E, WB
Buffer
HAP1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-721P - HAP1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_003940.2, NP_817084.1
Short Description
(IN) HAP1
This product belongs to the following categories:
Related Products
References- Takeshita Y, Fujinaga R, Zhao C, Yanai A, Shinoda K. Huntingtin-associated protein 1 (HAP1) interacts with androgen receptor (AR) and suppresses SBMA-mutant-AR-induced apoptosis. Hum Mol Genet. 2006 Aug 1;15(15):2298-312. Epub 2006 Jun 16.
Datasheet 08-01W
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