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Primary Antibodies
HACE1 Antibody
Background
HACE1 contains 6 ANK repeats and 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. HACE1 is an E3 ubiquitin-protein ligase that may function in cellular proteins degradation.
Additional Names
HACE1
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Description
Left: Western blot analysis of HACE1 in Hela cell lysate using HACE1 antibody at 1 μg/ml (6-18% gel).
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Purification
Antibody is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
HACE1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HACE1.
Application
HACE1 antibody can be used for detection of HACE1 by ELISA at 1:1562500. HACE1 antibody can be used for detection of HACE1 by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store HACE1 antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M, R
Protein Accession Number
NP_065822
Molecular Weight
102kDa
Short Description
HACE1 (HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1)
This product belongs to the following categories:
References- Zhang,L., (2007) Nat. Med. 13 (9), 1060-1069.
Datasheet 10-01W
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