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Primary Antibodies
HAAO Antibody
Background
HAAO (3-Hydroxyanthranilate 3,4-dioxygenase) is a monomeric cytosolic protein of the family of intramolecular dioxygenases containing non-heme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is present in low amounts in the central nervous system. This enzyme participates in tryptophan metabolism. It employs one cofactor, iron. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurological and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. Furthermore, recent study shows that HAAO are excellent candidate biomarkers for detecting ovarian cancer.
Additional Names
HAAO, 3-hydroxyanthranilate 3,4-dioxygenase, 3-hydroxyanthranilate oxygenase, 3-HAO, HAD, HAO
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Description
Left: Western blot analysis of HAAO in Mouse liver tissue lysate with HAAO antibody at 1 μg/ml.
Below: Immunohistochemistry of HAAO in human liver tissue with HAAO antibody at 2.5 μg/ml.
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Other Product Images
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Source
HAAO antibody was raised against a 17 amino acid peptide near the amino terminus of human HAAO.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
HAAO antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
HAAO antibody can be used for detection of HAAO by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5251P - HAAO Peptide
Storage
HAAO antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1404 - Mouse Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
188497625
Protein Accession Number
NP_036337
Short Description
3-hydroxyanthranilate 3,4-dioxygenase
This product belongs to the following categories:
References- Decker RH, Kang HH, Leach FR, et al. Purification and properties of 3-hydroxyanthranilic acid oxidase. J. Biol. Chem. 1961; 236:3076–82.
- Huang YW, Jansen RA, Fabbri E, et al. Identification of candidate epigenetic biomarkers for ovarian cancer detection. Oncol. Rep. 2009; 22:853-61.
Datasheet 09-01W
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