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Detection Sets
Grik Detection Set
Background
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and Grik5/KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Grik2, also known as glutamate receptor 6, may be associated with autosomal recessive mental retardation and possibly other neurological disorders such as schizophrenia. Numerous isoforms of Grik2 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow. Grik3, also known as glutamate receptor 7, has recently been shown to be an essential subunit of presynaptic kainate autoreceptors at hippocampal mossy fiber synapses as grik3-null mice show significantly reduced short- and long-term synaptic potentiation. Grik4 codes for the KA1 subunit of kainate-type ionotropic gluatamate receptors; mutations in this gene show significant association with both schizophrenia and bipolar disorder. Grik5, also known as kainate-preferring glutamate receptor subunit KA2, does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface.
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Description
For images please see PDF data sheet
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Kit Contents
Grik1 Antibody (IN), Catalog No. 4381 (50μg)
Grik2 Antibody (C2), Catalog No. 4387 (50μg)
Grik3 Antibody, Catalog No. 4389 (50μg)
Grik4 Antibody (CT), Catalog No. 4391 (50μg)
Grik5 Antibody, Catalog No. 4397 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of Grik1 – 5 by immunoblot at 1 – 2 μg/ml, and for detection of Grik1, 4 or 5 by immunohistochemistry at 2.5 μg/ml.
Tested Application
WB, IHC
Blocking Peptide
Grik1 Peptide (IN), Catalog No. 4381P
Grik2 Peptide (C2), Catalog No. 4387P
Grik3 Peptide, Catalog No. 4311P
Grik4 Peptide (CT), Catalog No. 4391P
Grik5 Peptide, Catalog No. 4397P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- Grik1 Antibody: Rat Brain Lysate, Catalog No. 1463
Grik2 Antibody: Human Brain Lysate, Catalog No. 1303
Grik3 Antibody: Human Brain Tissue Lysate, Catalog No. 1303
Grik4 Antibody: Rat Brain Lysate, Catalog No. 1463
Species Reactivity
see datasheet
Short Description
Grik1 (IN), Grik2 (C2), Grik3, Grik4 (CT), Grik5
This product belongs to the following categories:
Related Products
References- Tanaka K. Functions of glutamate transports in the brain. Neurosci. Res. 2000; 37:15-9.
- Pinheiro P and Mulle C. Kainate receptors. Cell Tissue Res. 2006; 326:457-82.
- Bureau I, Dieudonne S, Coussen F, et al. Kainate receptor-mediated responses in the CA1 field of wild-type and GluR6-deficient mice. J. Neurosci. 1999; 19:653-63.
- Christensen JK, Paternain AV, Selak S, et al. A mosaic of functional kainate receptors in hippocampal interneurons. J. Neurosci. 2004; 24:8986-93.
Datasheet 0801-W
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