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Primary Antibodies
GSTP1 Antibody
Background
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism (i.e., the metabolism of environmental mutagens and carcinogens) and may play a role in susceptibility to cancer. More recent experiments have suggested that differential expression of GSTP1 also contributes to the sensitivity of xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders such as Parkinson’s disease. CpG island hypermethylation of the GSTP1 promoter leading to the silencing of the GSTP1 gene has also been linked to cancer.
Additional Names
GSTP1, Glutathione S-transferase pi
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Description
Left: Western blot analysis of GSTP1 in Jurkat cell lysate with GSTP1 antibody at (A) 0.5 and (B) 1 μg/ml.
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Source
GSTP1 antibody was raised against a 14 amino acid peptide from near the center of human GSTP1.
Clonality / Clone
This is a polyclonal antibody.
Host
GSTP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
GSTP1 antibody can be used for the detection of ATG10 by Western blot at 0.5 – 1 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
GSTP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H,M,R
Protein Accession Number
NP_000843
Data Sheet Version
08-02W
Related Products
References
- Pearson WR. Phylogenies of glutathione transferase families. Methods Enzymol. 2005; 401:186-204.
- Clapper ML. Genetic polymorphism and cancer risk. Curr. Oncol. Rep. 2000; 2:251-6.
- Smeyne M, Boyd J, Shepherd KR, etc. GSTpi expression mediates dopaminergic neuron sensitivity in experimental parkinsonism. Proc. Natl. Acad. Sci. USA 2007; 104:1977-82.
- Ellinger J, Bastian PJ, Jurgan T, et al. CpG island hypermethylation at multiple gene sites in diagnosis and prognosis of prostate cancer. Urology 2008; 71:161-7.
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