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Catalog Number:

46-853


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

GPR142 Antibody

Background

GPR142 Antibody.

Additional Names

GPR142 , G protein-coupled receptor 142 , PGR2

GPR142 Antibody

Description

Left: Western blot analysis of GPR142 in human liver lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using GPR142 antibody (1μg/ml).

Source

GPR142 antibody was raised against a synthetic peptide of GPR142.

Purification

GPR142 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

GPR142 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 50-55kDa band observed in Human Liver lysates (calculated MW of 51.1kDa according to NP_861455.1). Recommended concentration: 1-3μg/ml.

Tested Application

E, WB

Buffer

GPR142 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 46-853P - GPR142 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Species Reactivity

H

Protein Accession Number

NP_861455.1

Short Description

GPR142

This product belongs to the following categories:

Related Products

References

  1. Süsens U, Hermans-Borgmeyer I, Urny J, Schaller HC. Characterisation and differential expression of two very closely related G-protein-coupled receptors, GPR139 and GPR142, in mouse tissue and during mouse development. Neuropharmacology. 2006 Mar;50(4):512-20. Epub 2005 Dec 27.


Datasheet 09-01W

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