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Primary Antibodies
GPAT1 Antibody
Background
Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.
Additional Names
GPAT1, Glycerol-3-phosphate acyltransferase 1, GPAM
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Description
Left: Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
GPAT1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human GPAT1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
GPAT1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4613P - GPAT1 Peptide
Storage
GPAT1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
190358539
Protein Accession Number
NP_065969
Short Description
Glycerol-3-phosphate acyltransferase 1
This product belongs to the following categories:
Related Products
References- Coleman RA and Lee DP. Enzymes of triacylglycerol synthesis and their regulation. Prog. Lipid Res. 2004; 43:134-76.
- Linden D, William-Olsson L, Ahnmark A, et al. Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation. FASEB J. 2006; 20:434-43.
- Eberle D, Hegarty B, Bossard P, et al. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004; 86:839-48.
- Lewin TM, de Jong H, Schwerbrock NJ, et al. Mice deficient in glycerol-3-phosphate acyltransferase-1 have diminished myocardial triacylglycerol accumulation during lipogenic diet and altered phospholipid fatty acid composition. Biochim. Biophys. Acta 2008; 1781:352-8.
Datasheet 08-03W
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