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Primary Antibodies
GOLPH2 Antibody
Background
GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer’s disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.
Additional Names
GOLPH2, Golgi phosphoprotein 2, golgi membrane protein 1, GOLM1, GP73
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Description
Left: Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/ml.
Below: Immunohistochemistry of GOLPH2 in rat brain tissue with GOLPH2 antibody at 2.5 μg/ml.
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Other Product Images
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Source
GOLPH2 antibody was raised against an 18 amino acid peptide from near the center of human GOLPH2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
GOLPH2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 – 0.5 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5441P - GOLPH2 Peptide
Storage
GOLPH2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
119583109
Protein Accession Number
EAW62705
Short Description
Golgi phosphoprotein 2
This product belongs to the following categories:
References- Kladney RD, Bulla GA, Guo L, et al. GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 2000; 249:53-65.
- Li H, Wetten S, Li L, et al. Candidate single-nucleotide polymorphisms from a genomewide association study of Alzheimer disease. Arch. Neurol. 2008; 65:45-53.
- Antunez C, Boada M, Lopez-Arrieta, et al. GOLPH2 gene markers are not associated with Alzheimer’s disease in a sample of the Spanish population. J. Alzheimers Dis. 2009; epub.
- Kristiansen G, Fritzsche FR, Wassermann K, et al. GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br. J. Cancer 2008; 99:939-48.
Datasheet 10-01W
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