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Primary Antibodies
GFR alpha 2 Antibody
Background
Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN), play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRα, which is a cell surface receptor for NTN. The cDNAs encoding NTNRa from human, rat, chicken, and mouse have been cloned recently. NTNRα was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFβ-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
Additional Names
GFR alpha 2 (IN), GFRa-2, GDNFRb, GDNFRbeta
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Description
Left: Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1:1000 dilution.
Below: Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/ml.
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Other Product Images
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Source
GFR alpha 2 antibody was raised against a peptide corresponding to amino acids 377 to 391 of human GFR alpha 2 The amino acid sequences of immunogenic peptide are identical to those of mouse and rat.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
GFR alpha 2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human GFR alpha 2 Peptide (Cat. No. 1135P)
Application
GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1:1000 to 1:2000 dilution. A 52 kDa band should be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1135P - GFR alpha 2 Peptide
Storage
GFR alpha 2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
2145080
Protein Accession Number
AAC52036
Short Description
(IN) Neurturin receptor
This product belongs to the following categories:
Related Products
References- Kotzbauer PT, Lampe PA, Heuckeroth RO, Golden JP, Creedon DJ, Johnson EM Jr, lbrandt J. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470
- Klein RD, Sherman D, Ho WH, Stone D, Bennett GL, Moffat B, Vandlen R, Simmons L, Gu Q, Hongo JA, Devaux B, Poulsen K, Armanini M, Nozaki C, Asai N, Goddard A, Phillips H, Henderson CE, Takahashi M, Rosenthal A. A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997;387:717-721
- Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A, Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724
- Baloh RH, Tansey MG, Golden JP, Creedon DJ, Heuckeroth RO, Keck CL, Zimonjic DB, Popescu NC, Johnson EM Jr, Milbrandt J. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997;18:793-802
Datasheet 08-02W
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