|
Primary Antibodies
GFR alpha 1 Antibody
Background
Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRa, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRα from human, rat, chicken and mouse have been cloned recently. GDNFRα was also termed Ret ligand 1 (RETL1) or TGF-β-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Additional Names
GFR alpha 1 (IN), GFR alpha 1, GFRa-1, GDNFRa, GDNFRalpha
|
Description
Left: Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1:500 dilution.
Below: Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 μg/ml.
|
|
Other Product Images
|
Source
GFR alpha 1 antibody was raised against a peptide corresponding to amino acids 369 to 382 of human GFR alpha 1.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
GFR alpha 1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human GFR alpha 1 (Intermediate Domain) Peptide (Cat. No. 1133P)
Application
GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot. The amino acid sequence of immunogenic peptide is identical to those of mouse and rat and the antibody recognizes GFR alpha 1 from human, mouse and rat origins.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1133P - GFR alpha 1 Peptide
Storage
GFR alpha 1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
- Cat. No. 1304 - Human Liver Tissue Lysate
- Cat. No. 1305 - Human Kidney Tissue Lysate
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
20141405
Protein Accession Number
P56159
Short Description
(IN) GDNF receptor
This product belongs to the following categories:
Related Products
References- Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z; Cupples R; Louis JC; Hu S; Altrock BW; Fox GM. GDNF-induced activation of the Ret protein tyrosien kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell, 1996;85:1113-24.
- Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD; Gray C; Armanini MP; Pollock RA; Herti F; Phillips HS; Goddard A; Moore MW; Buj-Bello A;Davies AM; Asai N; Takahashi M; Vandlen R; Henderson CE; Rosenthal A. Characterization of a multicomponent receptor for GDNF. Nature, 1996;382:80-83.
- Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C, Walus L, Robinson S, Jaworski G, Wei H, Tizard R, Whitty A, Pepinsky RB, Cate RL. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci U S. 1997;94:6238-6243
- Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A, Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724
Datasheet 08-02W
|
|
