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Primary Antibodies
GCLC Antibody
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene for catalytic subunit (GLCLC) encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p21-p22. GLCLC deficiency in human is associated with enzymopathic hemolytic anemia.
Additional Names
GCLC, EC 6.3.2.2, GAMMA-ECS, GCS, GLCL, GLCLC
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Description
Left: Immunohistochemistry staining of GCLC in adrenal (formalin-fixed paraffin-embedded) using GCLC antibody.
Below: Immunohistochemistry staining of GCLC in testis (formalin-fixed paraffin-embedded) using GCLC antibody.
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Other Product Images
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Purification
Protein A Column
Clonality / Clone
This is a polyclonal antibody.
Host
GCLC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
GCLC antibody was raised against amino acids 295-313 of GCLC. (Rat)
Application
GCLC antibody can be used for detection of GCLC by WB (2 - 5 μg/ml), IP (10 μg/mg lysate), IHC-parafin (5 μg/ml).
Tested Application
WB, IHC, IP
Buffer
GCLC antibody is supplied in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Storage
GCLC antibody can be stored at 4˚C or -20˚C, Avoid repeated freezing and thawing.
Species Reactivity
H, M, Mk
Formulation
Weight: 50 μg
Volume: 250 μl
Concentration: 0.2 mg/ml
Short Description
Glutamate-cysteine Ligase
This product belongs to the following categories:
Datasheet 09-01W
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