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Catalog Number:

45-659


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GeneDireX Molecular Weight Marker


Antibody Production


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Primary Antibodies

GBX2 Antibody

Background

GBX2 Antibody.

Additional Names

GBX2, gastrulation brain homeobox 2, gastrulation brain homeo box 2

GBX2 Antibody

Description

Left: Western blot analysis of GBX2 in Jurkat cell lysate (35μg protein in RIPA buffer) using GBX2 antibody (0.1μg/mL).

Source

GBX2 antibody was raised against a synthetic peptide of GBX2.

Purification

GBX2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

GBX2 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approx 37kDa band observed in lysates of T-cell line Jurkat (calculated MW of 37.3kDa according to NP_001476.2). Recommended concentration: 0.1-0.3μg/ml.

Tested Application

E, WB

Buffer

GBX2 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 45-659P - GBX2 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Positive Control

  1. Cat. No. 1205 - Jurkat Cell Lysate

Species Reactivity

H, M, R, D

Protein Accession Number

NP_001476.2

Short Description

GBX2

This product belongs to the following categories:

Related Products

References

  1. Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80.


Datasheet 08-01W

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