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Primary Antibodies
GBL Antibody
Background
GβL (G protein beta protein subunit-like) is a member of a signaling pathway that regulates mammalian cell growth in response to the presence of nutrients and growth factors (1). It binds to the kinase domain of TOR (Target of rapamycin, also known as mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3). TOR is normally associated with GβL and an additional regulatory protein RAPTOR, allowing TOR to control protein biosynthesis (3). The binding of GβL to TOR stimulates TOR’s kinase activity towards downstream proteins such as RPS6K (ribosomal protein S6 kinase) and the translation factor 4E-BP1 which leads to increased protein translation and cell growth (3).
Additional Names
GBL, G beta protein subunit-like
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Description
Left: Western blot analysis of GbL in human brain cell lysate with GbL antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of GbL in mouse brain tissue with GbL antibody at 10 μg/ml.
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Other Product Images
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Source
GbL antibody was raised against a 14 amino acid peptide from near the carboxy-terminus of human GbL.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
GBL antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human GbL / G beta protein subunit-like Peptide (Cat. No. 3495P)
Application
GbL antibody can be used for the detection of GbL by Western blot at 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3495P - GBL Peptide
Storage
GBL antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
- Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
30411038
Protein Accession Number
AAH52292
Short Description
a member of the TOR signaling pathway
This product belongs to the following categories:
Related Products
References- Kim D-H, Sarbassov DD, Ali SM, et al. GβL, a positive regulator of the Rapamycin-sensitive pathway required for the nutrient-sensitive interaction between Raptor and mTOR. Mol. Cell 2003; 11:895-904.
- Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
- Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.
Datasheet 08-02W
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