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Primary Antibodies
GBL Antibody
Background
GBL protein contains seven WD-40 repeats, which are common to G-protein β subunits (Gβ). Although chemically and structurally similar to Gβ subunits, the amino acid sequence was found to be only 31-41% similar and thus was named Gβ-like. Expression of GBL mRNA was highest in the brain and testis, followed by lung, heart, kidney, skeletal muscle, spleen and liver. The ubiquitous nature of the tissue expression pattern of GBL is similar to that of the insulin receptor, which suggests that insulin may influence GBL expression. GBL protein and mRNA levels, in fully differentiated 3T3-L1 adipocytes, were upregulated by insulin in a concentration-dependent fashion. These changes were highly sensitive to insulin stimulation, being minimally affected by doses as low as 0.1 nM and maximally elevated by 1 nM doses. These data suggest that insulin regulates GBL production and imply that some of the actions of insulin may be mediated, in part, by this intracellular protein.
Additional Names
G protein b subunit
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Description
Left: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
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Location
The gene that codes for this protein is at chromosomal location 16p13.3.
Purification
Immunoaffinity Purified
Clonality / Clone
This is a polyclonal antibody.
Host
GBL antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Immunogen
20-270
Application
G protein β subunit antibody can be used for the detection of G protein β subunit by Western Blot.
Tested Application
WB
Buffer
Phosphate-Buffered Saline. No preservatives added.
Storage
GBL antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M, R
Protein GI Number
21361908
Protein Accession Number
NP_071767.2
Molecular Weight
25.4 kDa (calculated)
Short Description
G protein b subunit
This product belongs to the following categories:
Datasheet 0801-W
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