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Primary Antibodies
GATA3 Antibody
Background
GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.
Additional Names
GATA3, GATA binding protein 3, HDR
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Description
Left: Western blot analysis of GATA3 in human brain tissue lysate with GATA3 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 μg/ml.
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Other Product Images
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Source
GATA3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human GATA3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
GATA3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5599P - GATA3 Peptide
Storage
GATA3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
50541959
Protein Accession Number
NP_001002295
Short Description
GATA binding protein 3
This product belongs to the following categories:
References- Ko LJ, Yamamoto M, Leonard MW, et al. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol. Cell. Biol. 1991; 11:2778-84.
- Ma GT, Roth ME, Groskopf JC, et al. GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo. Dev. 1997; 124:907-14.
- Home P, Ray S, Dutta D, et al. GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J. Biol. Chem. 2009; 284:28729-37.
- Strumpf D, Mao CA, Yamanaka Y, et al. CDX2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Dev. 2005; 132:2093-102.
Datasheet 10-01W
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