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Primary Antibodies
GAPDH Antibody
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Additional Names
GAPDH (NT), Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
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Description
Left: Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.125, (B) 0.25 and (C) 0.5 μg/ml.
Below: Immunohistochemistry of GAPDH in human liver tissue with GAPDH antibody at 10 μg/ml.
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Other Product Images
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Source
GAPDH antibody was raised against a 16 amino acid peptide from near the amino-terminus of human GAPDH.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
GAPDH antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human GAPDH / Glyceraldehyde-3-phosphate dehydrogenase / G3PDH / GAPD (N-Terminus) Peptide (Cat. No. 3783P)
Application
GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 - 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3783P - GAPDH Peptide
Storage
GAPDH antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
120649
Protein Accession Number
P04406
Short Description
(NT) An eznyme in the glycolytic pathway
This product belongs to the following categories:
Related Products
References- Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
- Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
Datasheet 08-02W
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