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Primary Antibodies
GABRB3 Antibody
Background
GABRB3 Antibody. This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1).
Additional Names
GABRB3, gamma-aminobutyric acid (GABA) A receptor, beta 3, MGC9051, GABA(A) receptor beta-3 subunit, GABA-A receptor beta-3 subunit, GABA-alpha receptor beta-2 subunit, GABAA receptor beta-3 subunit
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Description
Left: Western blot analysis of GABRB3 in Human Placenta lysate (35μg protein in RIPA buffer) using GABRB3 antibody (0.1μg/ml).
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Source
GABRB3 antibody was raised against a synthetic peptide of GABRB3.
Purification
GABRB3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
GABRB3 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1μg/ml).
Tested Application
E
Buffer
GABRB3 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-774P - GABRB3 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1309 - Human Placenta Lysate
Species Reactivity
H, M, R, D, B
Protein Accession Number
NP_000805.1, NP_068712.1
Short Description
GABRB3
This product belongs to the following categories:
Related Products
References- Tanaka M, Olsen RW, Medina MT, Schwartz E, Alonso ME, Duron RM, Castro-Ortega R, Martinez-Juarez IE, Pascual-Castroviejo I, Machado-Salas J, Silva R, Bailey JN, Bai D, Ochoa A, Jara-Prado A, Pineda G, Macdonald RL, Delgado-Escueta AV. Hyperglycosylation and reduced GABA currents of mutated GABRB3 polypeptide in remitting childhood absence epilepsy. Am. J. Hum. Genet. 2008 Jun 82 (6): 1249-61.
Datasheet 09-01W
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