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Primary Antibodies
FasL (MAb H11) (FITC) Antibody
Background
*Please allow 3-5 days for delivery time.*
Additional Names
CD95L, CD178, TNFSF 6
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Description
The immunogen is a synthetic peptide corresponding to amino acids 196-220 of mouse FasL (CD95L; APO-1L; CD178). The schematic structure of mouse FasL (peptide, aa. 196-220) as seen in this image.
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Source
FasL (monoclonal H11) antibody was raised against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
Purification
FasL (monoclonal H11) antibody was purified to ≥95% in SDS-PAGE and is supplied at a concentration of 1mg/1ml. This product is biotin conjugated.
Clonality / Clone
This is a monoclonal antibody.
Isotype
Rat IgG2a
Host
FasL antibody was raised in rat.
Please use anti-rat IgG secondary antibodies
Immunogen
The immunogen is a synthetic peptide corresponding to amino acids 196-220 of mouse FasL (CD95L; APO-1L; CD178).
Application
FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunocytochemistry. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 µg/ml of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Method: Cells extracts from (2 x 106) cells transfected with a FasL-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL H11 antibody at 2µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system. Arrow indicates FasL. Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg of FITC-labeled H11 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition (see T. Renno, M. Hahne, J. Tschopp and H. R. MacDonald, J. Exp. Med., 183, 431 (1996)). Note: Activated primary T cells do not express high levels of surface FasL! This product is for research use only.
Tested Application
IHC, FC
Buffer
FasL antibody is supplied as a liquid in PBS containing 0.02% sodium azide.
Storage
FasL antibody can be stored at 4˚C, stable for one year.
Species Reactivity
M
Short Description
Fluorescein - monoclonal to FasL (mouse) (H11)
This product belongs to the following categories:
References- Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995).
- CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179, 153 (1997).
- Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167, 1338 (2001).
Datasheet 0802-W
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