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Primary Antibodies
FTO Antibody
Background
Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Additional Names
FTO, Fat mass and obesity associated
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Description
Left: Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 μg/ml.
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Other Product Images
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Source
FTO antibody was raised against a 15 amino acid peptide from near the amino terminus of human FTO.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
FTO antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5137P - FTO Peptide
Storage
FTO antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1317 - Human Uterus Tissue Lysate
Species Reactivity
H
Protein GI Number
148841515
Protein Accession Number
Q9C0B1
Short Description
Fat mass and obesity associated protein
This product belongs to the following categories:
References- Scuteri A, Sanna S, Chen W-M, et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PloS Genet. 2007; 3:e115.
- Gerken T, Girard CA, Tung YCL, et al. The obesity-associated FTO gene encodes a 2-oxyglutarate-dependent nucleic acid demethylase. Science 2007; 318:1469-72.
- Fredriksson R, Hagglund M, Olszewski PK, et al. The obesity gene, FTO, is of ancient origin, upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology 2008; 149:2062-71.
- Stratigopoulous G, Padilla S, Leduc CA, et al. Regulation of FTO/FTM gene expression in mice and humans. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2008; 294:R1185-96.
Datasheet 09-01W
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