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Primary Antibodies
FTO Antibody
Background
FTO Antibody.
Additional Names
Fto, fat mass and obesity associated, AW743446, mKIAA1752, similar to FTO
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Description
Left: Western blot analysis of FTO in human pancreas lysate (35μg protein in RIPA buffer) using FTO antibody (0.03μg/mL).
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Source
FTO antibody was raised against a synthetic peptide of FTO.
Purification
FTO antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
FTO antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approx. 60kDa band observed in human pancreas lysates (calculated MW of 58.0kDa according to NP_036066.2). Recommended concentration: 0.03-0.1μg/ml.
Tested Application
E, WB
Buffer
FTO antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-733P - FTO Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1307 - Human Pancreas Tissue Lysate
Species Reactivity
H, M, R, D, P
Protein Accession Number
NP_036066.2
Short Description
FTO (mouse)
This product belongs to the following categories:
Related Products
References- Gerken T, Girard CA, Tung YC, Webby CJ, Saudek V, Hewitson KS, Yeo GS, McDonough MA, Cunliffe S, McNeill LA, Galvanovskis J, Rorsman P, Robins P, Prieur X, Coll AP, Ma M, Jovanovic Z, Farooqi IS, Sedgwick B, Barroso I, Lindahl T, Ponting CP, Ashcroft FM, O'Rahilly S, Schofield CJ. The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase. Science. 2007 Nov 30;318(5855):1469-72. Epub 2007 Nov 8.
Datasheet 09-01W
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