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Primary Antibodies
FMR1 Antibody
Background
FMR1 Antibody.
Additional Names
FMR1, fragile X mental retardation 1 , FMRP, FRAXA, MGC87458
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Description
Left: Western blot analysis of FMR1 in KELLY cell lysate (35μg protein in RIPA buffer) using FMR1 antibody (0.1μg/ml).
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Source
FMR1 antibody was raised against a synthetic peptide of FMR1.
Purification
FMR1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
FMR1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 80kDa band observed in lysates of cell line KELLY (calculated MW of 71.2kDa according to NP_002015.1). The observed molecular weight corresponds to earlier findings in litera
Tested Application
E, WB
Buffer
FMR1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-842P - FMR1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R, D
Protein Accession Number
NP_002015.1
Short Description
FMR1
This product belongs to the following categories:
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References- Hanson JE, Blank M, Valenzuela RA, Garner CC, Madison DV. The functional nature of synaptic circuitry is altered in area CA3 of the hippocampus in a mouse model of Down's syndrome. J Physiol. 2007 Feb 15;579(Pt 1):53-67. Epub 2006 Dec 7.
Datasheet 09-01W
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