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Primary Antibodies
FLIP Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPα, β, γ, and δ, respectively (8).
Additional Names
FLIP (CT), I-FLICE
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Description
Left: Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.
Below: Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 μg/ml.
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Other Product Images
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Source
FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
FLIP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bacteria FLIP (C-Terminus) Peptide (Cat. No. 1161P)
Application
Flip antibody can be used for detection of FLIP by Western blot at 1:500 to 1:2000 dilution. A 55 kDa band can be detected. antibody recognizes the FLIPa only.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1161P - FLIP Peptide
Storage
FLIP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
2253679
Protein Accession Number
AAC51622
Short Description
(CT) FLICE inhibitory protein
This product belongs to the following categories:
Related Products
References- Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521
- Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195
- Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763
- Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257
Datasheet 08-02W
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